SCGE:18000000119 (Project)

Id Namespace:

Somatic Cell Genome Editing

Identifier Namespace (Entity type)

Dcc Asset:

scge cfde submission 20251016 143304.zip

Processed File (Entity type)

Dcc:

SCGE

DCC (Entity type)

Persistent Id:https://scge.mcw.edu/toolkit/data/experiments/experiment/18000000119

Description:WT female mouse and homozygous male mouse containing the Traffic Light Reporter 2 (TLR2) reporter at the Rosa26 locus were mated. Females were super-ovulated and zygotes harvested. Zyotes were electroporated with Cas9 RNP along with test guides and were cultured 96 hours to blastocyst stage. Blastocysts were imaged for tagRFP fluorescence and scored for fluorescence signal. Blastocysts were then PCR amplified for the reporter allele and Sanger sequenced. ICE analysis tool from Synthego was used to score edits. NOTE: Some blasts did not PCR amplify well and it could not be determined if they had edits. Total blasts analyzed expressing the fluorescent reporter, 46-120. Total blast analyzed by Sanger sequencing, 8-64.

Abbreviation:SCGE18000000119

Results found

Linked to

	Label	Description
	SCGE:1081 Project (Entity type)	SCGE study 1081
	SCGE:15000005227 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000119 project
	SCGE:15000005228 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000119 project
	SCGE:15000005226 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000119 project
	SCGE:15000005225 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000119 project
	SCGE:15000005229 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000119 project
	SCGE:15000005230 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000119 project

SCGE:1081

Project (Entity type)

SCGE study 1081

SCGE:15000005227

Biosample (Entity type)