PR001733 (Project)

Id Namespace:

UCSD Metabolomics Workbench

Identifier Namespace (Entity type)

Dcc Asset:

MW submission packet 20251214.zip

Processed File (Entity type)

Dcc:

Metabolomics

DCC (Entity type)

Persistent Id:http://dx.doi.org/10.21228/M8PX3J

Description:We performed targeted metabolomic analysis on the Spemann-Mangold Organizer (SMO) tissue in the frog (Xenopus laevis) and the remainder of dissected embryos (RE). The goal of this study is to quantify a panel of targeted metabolite intermediates from glycolysis, phosphate energy pool, and mitochondrial activity including the TCA cycle. Metabolites were extracted from the dissected tissues, reconstituted, and analyzed using liquid chromatography (LC) electrospray ionization (ESI) mass spectrometry (MS). The targeted metabolite measurements were performed on a trapped ion mobility time-of-flight mass spectrometer (timsTOF PRO, Bruker). Targeted MS assays on the metabolite intermediates produced downstream, complemented by classical fluorescence-based metabolite assays when available, revealed local oxidative stress and enrichment of reactive oxygen species (ROS) in the SMO.

Results found

Linked to

	Label	Description
	PPR00001 Project (Entity type)	Metabolomics
	SU002885 Subject (Entity type)	A subject from Metabolomics produced as part of the PR001733 project
	ST002778 Organizer metabolomics rawdata.zip File (Entity type)	A 1.25 GB file from Metabolomics produced from OBI:0003097 as part of the PR001733 project
	SA297339 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001733 project
	SA297369 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001733 project
	SA297357 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001733 project
	SA297445 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001733 project
	SA297353 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001733 project
	SA297385 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001733 project
	SA297447 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001733 project

PPR00001

Project (Entity type)

Metabolomics

SU002885

Subject (Entity type)

A subject from Metabolomics produced as part of the PR001733 project

ST002778 Organizer metabolomics rawdata.zip

File (Entity type)

A 1.25 GB file from Metabolomics produced from OBI:0003097 as part of the PR001733 project

SA297339

Biosample (Entity type)