PR000998 (Project)

Id Namespace:

UCSD Metabolomics Workbench

Identifier Namespace (Entity type)

Dcc Asset:

MW submission packet 20250922.zip

Processed File (Entity type)

Dcc:

Metabolomics

DCC (Entity type)

Persistent Id:http://dx.doi.org/10.21228/M8PQ57

Description:The integration of circadian and metabolic signals is essential for maintaining robust circadian rhythms and ensuring efficient metabolism and energy use. Using Drosophila as an animal model, we showed observed strong correlation between daily daily rhythms of protein O- linked N-acetylglucosaminylation (O-GlcNAcylation) and clock-controlled feeding-fasting cycles, suggesting that O-GlcNAcylation rhythms are primarily driven by nutrient input. Interestingly, daily O-GlcNAcylation rhythms were severely dampened when we subjected flies to time- restricted feeding (TRF) at unnatural feeding time. This suggests the presence of a clock- regulated buffering mechanism that prevents excessive O-GlcNAcylation at non-optimal times of the day-night cycle, which could disrupt circadian health. We performed targeted metabolomic analysis on hexosamine biosynthetic pathway (HBP), which produces UDP-GlcNAc (the substrate for O-GlcNAcylation), to evaluate the daily activity of HBP enzymes under TRF conditions. We found glutamine--fructose-6-phosphate amidotransferase (GFAT) mediates this buffering mechanism.

Results found

Linked to

	Label	Description
	PPR00001 Project (Entity type)	Metabolomics
	SU001526 Subject (Entity type)	A subject from Metabolomics produced as part of the PR000998 project
	SA124094 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000998 project
	SA124113 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000998 project
	SA124091 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000998 project
	SA124086 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000998 project
	SA124067 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000998 project
	SA124102 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000998 project
	SA124052 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000998 project
	SA124063 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000998 project