PR002259 (Project)

Description:Over 100 types of adenoviruses exist across 7 species, with about 50 known to infect humans. Human adenoviruses overtake the DNA replication machinery of the infected host, rewiring mitotic events. Infection leads to the elevations in glucose and glutamine consumption rates, while also increasing lactate production rate. Fibroblast lineages are normally quiescent cells that display a repertoire of responses to certain agonists. Data on the shifts in fibroblast metabolism in response to human adenoviral infection are lacking. Specifically, knowledge pertaining to metabolic shifts aside from those involved in glycolytic metabolism after human adenoviral infection remains sparse. We used an untargeted metabolomic approach to better understand the dynamic metabolic changes to fibroblasts in response to 3 viral dosages, across 4 time points post-infection. Profound shifts were seen in cysteine, purine, and unsaturated fatty acid metabolites. This analysis provides a global perspective and highlights previously underappreciated aspects of how human adenovirus alters host metabolism.