PR001645 (Project)

Description:Globally, the incidence of chronic kidney disease is increasing, raising serious concerns about its impact on public health. It also poses significant difficulty in finding novel early diagnostics, understanding biochemical pathways, monitoring patients, and prognosis. Any metabolite found in a biofluid, or tissue may act as a driver, signal, or both in the emergence or spread of the disease. As a result, metabolomics is a very useful strategy in this therapeutic setting. Broad metabolite coverage is essential since it strives to offer a representative image of a biological system. An untargeted metabolomics-based method was used in this cross-sectional study to identify metabolomic changes and their relationship to pathways in the Arabic gum patient group and control participants. Plasma samples were collected from 88 participants who met the inclusion criteria, of whom 43 control patients were treated with a placebo and 45 intervention patients were treated with Arabic gum. Highly sensitive ultra-high-performance liquid chromatography with electrospray ionization and quadrupole time-of-flight mass spectrometry was used to analyze the plasma samples (UHPLC-ESI-QTOF-MS). We investigated the effect of Arabic gum on individual metabolites using a two-tailed independent student t-test. The results showed that 31 out of 92 identified metabolites were found to be statistically significant (p < 0.05). L-Leucine and 5'-Methylthioadenosine were the significantly increased metabolites in the Arabic gum group. Conversely, triethylamine, D-limonene, 4-methylphenylacetic acid, and sphingosine levels were significantly lower in the Arabic gum group compared to the control. Arabic gum primarily affected multiple metabolic pathways, including glycine and serine, arginine and proline, valine, leucine, and isoleucine degradation, phenylalanine and tyrosine, urea cycle, and sphingolipid. The results from this study provide insights into the potential diagnostic significance of different metabolites in chronic kidney disease and their impact on specific metabolic pathways. However, further research involving larger cohorts is necessary to validate the observed metabolite changes following Arabic gum intake and their diagnostic value for chronic kidney disease.