PR001337 (Project)

Id Namespace:

UCSD Metabolomics Workbench

Identifier Namespace (Entity type)

Dcc Asset:

MW submission packet 20250922.zip

Processed File (Entity type)

Dcc:

Metabolomics

DCC (Entity type)

Persistent Id:http://dx.doi.org/10.21228/M8WM52

Description:This metabolite dataset was collected from bacterial channel rhodopsin expressing transgenic mouse models subjected to optic nerve crush (ONC) with subsequent stimulation with light (promoting regeneration) or non-stimulation (lacking axon regeneration). ONC retains retinal ganglion cells within the retina, while degenerating axons. In transgenic bacterial channel rhodopsin expressing cells, light stimulation promotes regeneration. Genetically matched wild-type uninjured optic nerves, or control transgenic mice, were also analyzed. Metabolites were carefully extracted from finely minced optic nerve tissue using a solvent system (initial separation using 1:1 Methanol and H2O and second extraction using 8:1:1 of Acetonitrile:Acetone:Methanol). Untargeted liquid chromatography-mass spectrometry (LC-MS/MS) profiling was performed using fractionation on a Vanquish Horizon Binary UHPLC. Subsequent analyses were performed on an inline coupled Q-Exactive Orbitrap instrument. Metabolites were identified using Compound DiscovererTM software. Statistical analysis was performed using MetaboAnalyst 5.0.

Results found

Linked to

	Label	Description
	PPR00001 Project (Entity type)	Metabolomics
	SU002196 Subject (Entity type)	A subject from Metabolomics produced as part of the PR001337 project
	SA202449 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001337 project
	SA202491 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001337 project
	SA202494 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001337 project
	SA202448 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001337 project
	SA202490 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001337 project
	SA202481 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001337 project
	SA202452 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001337 project
	SA202478 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001337 project