Description:We performed high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of the lipidome of GC from C57BL/6 mice across five age groups (E18, P0, P3, P6, and P9) from two growth cone fractions: growth cone membrane (GCM) and growth cone particulate (GCP). These fractions were generated through established differential centrifugation techniques and lipid extraction. Developmental time points were chosen based upon our understanding of brain development in the murine model. In mice cortex, E18 is characterized by extension or elongation of axons toward targets, while around P0-P3 is when axons start innervating targets and branching occurs. At P6-P7 is when target innervation is generally complete and a subpopulation of cortical neurons undergo developmental cell death. By P9 growth cones have mostly disappeared.