SCGE:18000000121 (Project)

Id Namespace:

Somatic Cell Genome Editing

Identifier Namespace (Entity type)

Dcc Asset:

scge cfde submission 20251016 143304.zip

Processed File (Entity type)

Dcc:

SCGE

DCC (Entity type)

Persistent Id:https://scge.mcw.edu/toolkit/data/experiments/experiment/18000000121

Description:WT female mouse and homozygous male mouse containing the Traffic Light Reporter 2 (TLR2) reporter at the Rosa26 locus were mated. Females were super-ovulated and zygotes harvested. Zyotes were electroporated with Cas9 RNP along with test guides and were cultured 96 hours to blastocyst stage. Blastocysts were imaged for tagRFP fluorescence and scored for fluorescence signal. Blastocysts were then PCR amplified for the reporter allele and Sanger sequenced. ICE analysis tool from Synthego was used to score edits. NOTE: Total blasts analyzed expressing the fluorescent reporter, 55-67. Total blast analyzed by Sanger sequencing, 55-67.

Abbreviation:SCGE18000000121

Results found

Linked to

	Label	Description
	SCGE:1081 Project (Entity type)	SCGE study 1081
	SCGE:15000005234 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000121 project
	SCGE:15000005236 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000121 project
	SCGE:15000005235 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000121 project

SCGE:1081

Project (Entity type)

SCGE study 1081

SCGE:15000005234

Biosample (Entity type)

A biosample from SCGE produced as part of the SCGE:18000000121 project

SCGE:15000005236

Biosample (Entity type)

A biosample from SCGE produced as part of the SCGE:18000000121 project

SCGE:15000005235

Biosample (Entity type)

A biosample from SCGE produced as part of the SCGE:18000000121 project

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The CFDE Workbench is actively being developed and maintained by the CFDE Data Resource Center (DRC).The DRC is funded by OT2OD036435 from the Common Fund at the National Institutes of Health.

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