PR002613 (Project)

Id Namespace:

UCSD Metabolomics Workbench

Identifier Namespace (Entity type)

Dcc Asset:

MW submission packet 20250922.zip

Processed File (Entity type)

Dcc:

Metabolomics

DCC (Entity type)

Persistent Id:http://dx.doi.org/10.21228/M8WK0T

Description:Amongst the thousands of transport proteins constituting approximately 10% of coding sequences in a genome, only few have an assigned function. Heterologous expression of transporters in combination with the use of plant extracts as complex mixtures of substrates can be a powerful tool for untargeted identification of plant transporter functionality. In this study, we developed and evaluated four extraction protocols to generate Arabidopsis thaliana seedling extracts for use as substrate mixtures in high-throughput screening of transporters expressed in Xenopus laevis oocytes, a well-established system for transporter studies. Our study highlights the importance of developing metabolically diverse yet low-toxicity plant extracts as a critical step toward advancing plant transporter substrate screening. The optimized extraction protocol in combination with X. laevis oocyte assays provide a robust platform for the functional characterization of plant transporters, paving the way for deeper insights into plant physiology and metabolism.

Results found

Linked to

	Label	Description
	PPR00001 Project (Entity type)	Metabolomics
	SU004302 Subject (Entity type)	A subject from Metabolomics produced as part of the PR002613 project
	SA480484 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002613 project
	SA480441 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002613 project
	SA480530 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002613 project
	SA480503 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002613 project
	SA480407 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002613 project
	SA480409 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002613 project
	SA480533 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002613 project
	SA480545 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002613 project