Description:4DNES6V1ADE6: Multiplexed FISH of AAVS1 locus in HEK293T - rTetR-HDAC4 with doxycyline treatment for 5 days Multiplexed FISH in HEK293T reporter cell line treated with doxycyline for 5 days resulting in accumulation of HDAC4 causing silencing of reporter gene expression in cells (control and experiment relationships are described in detailed in 4DNFIWQ9QBOB supplementary file).