Description:In order to explore the metabolic differences between the two major traditional Chinese medicine disorders related to gallstones, namely "Liver Qi Stagnation" (LQS) and "Liver and Gallbladder Damp Heat" (LGDH), this study employed a comprehensive metabolomics approach on serum and urine. Our aim was to identify objective biomarkers in the serum and urine to achieve the differentiation of the disorders and to elucidate the underlying biological basis.