PR001388 (Project)

Id Namespace:

UCSD Metabolomics Workbench

Identifier Namespace (Entity type)

Dcc Asset:

MW submission packet 20251214.zip

Processed File (Entity type)

Dcc:

Metabolomics

DCC (Entity type)

Persistent Id:http://dx.doi.org/10.21228/M89H7H

Description:The emergence of multidrug resistance in Plasmodium falciparum parasites presents a significant obstacle to the malaria elimination agenda. Resistance to piperaquine (PPQ), an important first-line partner drug, has spread across Southeast Asia where it has contributed to widespread treatment failures. The genetic cause of resistance to PPQ is attributable to a novel set of amino acid substitutions in the P. falciparum chloroquine resistance transporter (PfCRT). In this study, we used magnetically-purified trophozoite extracts from seven different lines comprising five genetically-modified and two field isolates. Three independent extractions with triplicate technical repeats were run by mass spectrometry on positive and negative modes and spectral peak raw data analyzed to obtain the fold change difference between the samples and parental control, Dd2Dd2crt. We show that PPQ-resistant, PfCRT mutant asexual blood stage parasites accumulate higher levels of hemoglobin-derived peptides than do their PPQ-sensitive counterparts.

Results found

Linked to

	Label	Description
	PPR00001 Project (Entity type)	Metabolomics
	SU002267 Subject (Entity type)	A subject from Metabolomics produced as part of the PR001388 project
	ST002181 RawData.zip File (Entity type)	A 3.77 GB file from Metabolomics produced from OBI:0003097 as part of the PR001388 project
	ST002181 AN003573 Results.txt File (Entity type)	A 1.61 MB file from Metabolomics produced from OBI:0003097 as part of the PR001388 project
	ST002181 AN003572 Results.txt File (Entity type)	A 323 KB file from Metabolomics produced from OBI:0003097 as part of the PR001388 project
	SA209680 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001388 project
	SA209673 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001388 project
	SA209667 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001388 project
	SA209631 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001388 project
	SA209611 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001388 project