PR002060 (Project)

Description:We investigated the relationship between the host and gut microbiota in the context of Crohn Disease (CD), which is a relapsing-remitting condition. We analyzed paired omics of 80 CD patients and 43 controls, including 202 serum and 294 fecal metabolomics, and 258 microbiome samples. Our findings suggest that CD patients have an inverse shift in energy utilization from sugars and fat between serum and feces. In the serum, we noted a decrease in metabolites related to starch, sucrose, and tricarboxylic acid (TCA) cycle, as well as an increase in metabolites linked to fat in contrast to the feces, where we noted higher sugars and TCA cycle metabolites, and lower fat metabolites. Interestingly, fecal sugars were specifically linked with oral bacteria mislocated to the CD gut, while unsaturated fat derivatives of arachidonic acid were linked with R. gnavus and Fusobacteria. We identified consistent metabolite alterations in CD patients, which were also present in clinical/biomarkers active CD, and pre-flare samples of patients who experienced flare. Using pre-flare samples, we developed models that predicted a subsequent flare using metabolic serum and fecal signatures. We validated the fecal metabolomics predictions in another, similarly designed, independent CD cohort. Finally, we developed a clinical lab-based index [UA/Cr ratio+log2(CRP)] based on the serum metabolomics model, which was also predictive in the validation cohort. Here are the serum metabolomics samples.