PR002151 (Project)

Description:Using 13C-inulin as a tracer, we tracked the biosynthesis of polyamines in the human and mouse fecal microbiome. Additionally, single-strain SIRM analyses was used to explore functional gut microbes. Liquid chromatography-high resolution mass spectrometry analysis revealed distinct 13C enrichment profiles for polyamines. SIRM analyses were performed using a Q-Exactive HF mass spectrometer, equipped with an Ion Max API source and a HESI II probe, and were coupled to a Dionex UltiMate 3000 UHPLC system (Thermo Fisher Scientific). The findings revealed a novel de novo SPD biosynthesis pathway in the human gut microbiome, and Bacteroides (including B.fragilis and B.thetaiotaomicron) contributed to the biosynthesis of polyamines, underscoring the importance of polyamine bioanalysis in aligning gut microbial functions to host intestinal health.