Description:The goal of this project was to better understand Type 2 diabetes (T2D) at its earliest stages. The project followed 106 healthy and prediabetic subjects during periods of health, respiratory viral infection (RVI) and other perturbations. In one such perturbation, a subset of 23 individuals underwent a directed weight gain followed by weight loss. In total, 1,092 collections across all participants were profiled. Samples (primarily blood and stool) were collected quarterly and additional samples collected during periods of RVI and other stresses. The genomes of the participants were sequenced and measurements of transcriptomes, proteomes, metabolomes, and microbiomes taken at each visit, in addition to clinical details.