PR002416 (Project)

Description:Integrative multi-omics analysis of biological specimens such as tissues and biofluids (e.g., plasma and urine) is a powerful approach for gaining comprehensive understanding of the complex biological systems as well as in the identification of disease biomarkers. The great majority of omics datasets collected thus far resulted from a single omic (e.g. proteomics, metabolomics, lipidomics and others) study, which represents a challenge for any subsequent multiomics integration analysis. Filter Aided Sample Preparation (FASP) is a rapid and well-established technique for facilitating bottom-up proteomics preparation. However, FASP has only been employed in proteomics analysis and its utility for simultaneously isolating other biomolecules remains largely unexplored. This study assesses the performance of FASP as a convenient protocol to isolate protein and metabolite fraction from the same urine sample. Here FASP based LC-MS/MS analysis resulted in the identification of 3163 non-redundant peptides, 957 of which were unique protein groups. In parallel, LC-Qtof-MS and GC-MS/MS analysis of metabolites fraction obtained from urine solvent based extraction, FASP filtrate, FASP residue (concentrated protein) detected 145 metabolites by LC-MS and 139 metabolites by GC-MS. Our study demonstrates that the outcomes from FASP filtrate are comparable to those from solid phase extraction or FASP residue, in terms of both qualitative and quantitative analysis. Arguing that the proposed multiomics- FASP protocol should be considered for a multiomics single-step sample preparation analysis.