Description:4DNES9PJO3YW: SMSN (Barentine et al 2019 bioRxiv) - Lamin/nucleolus in U2OS Validation experiment of high-throughput 3D, two-color single-molecule switching nanoscopy. This experiment tested whether the fast speeds attained by our imaging platform were sustainable for over 24 hours, and additionally served as a test of large-scale SMS nanoscopy image analysis.