Description:4DNESMWANQOC: SMSN (Barentine et al 2019 bioRxiv) - DNA/mitochondria in U2OS Validation experiment of fast 3D, two-color single-molecule switching nanoscopy on relatively thin targets. This sample targets one structure (mitochondrial nucleoids) which is expected to be inside of the organelle targeted by the other probe (mitochondrial outer membrane).